• Task 1.1 – PED pipeline: technical task implying engineering activity
• Task 1.2 – SLiMs from structure: Detection of short linear motifs from structure is complicated. Simple pattern search generates lot of false positives (FPs) since they are very short (ca. 5 residues). The task will provide new strategies to limit FPs by filtering matches localized to buried residues outside linear structural stretches.
• Task 1.3 – IDP text-mining: Text-mining is widely used for retrieving functional data from the literature, but a feasibility study focused on intrinsic disorder is missing. The task will test different tools and methods to find the best solution applicable for large-scale automatic searches. This information will then be integrated into existing web server interfaces (WP4) to increase knowledge on experimental IDPs.
• Task 1.4 – IDR from structure: IDR detection from structural data depends on the type of source. Detection of elongated structures in protein multi-chain X-ray structures will be done by comparing intra- vs. inter-chain residue contacts and training the parameters on the ANCHOR dataset. Moreover, flexible regions will be calculated from B-factor information. Homology transfer is well established for structured domains but very problematic for disordered regions as IDP evolution seems to follow different rules.
• Task 1.5 – IDR homology transfer: will provide an extensive analysis of the parameters and constraints necessary to safely apply transfer by homology to IDPs based on sequence similarity.
• Task 1.6 – Mobi 2.0: Technical task implying engineering activity

Deliverables